Adaptive Immune System Analysis at Single-Cell Resolution

Sponsor: 10x Genomics

The new Chromium™ Single Cell V(D)J Solution allows the profiling of paired V(D)J transcripts from hundreds to millions of lymphocytes. The solution is optimized for the assembly of full V(D)J sequences—5’ untranslated region (UTR) to constant regions—from short-read sequencing on a cell-by-cell basis. Five-prime barcoding limits bias caused by complex multiplex PCR, enables the detection of germline and somatic variants across the entire V(D)J segment and supports cell-type classification or phenotyping. It includes necessary chemistry and microfluidics consumables as well as a complete software suite for analysis and visualization of large V(D)J datasets. Cell Ranger™ pipeline uses barcoding for gene expression analysis with single-cell resolution at million-cell scale. Loupe™ for Cells visualization application provides easy-to-use clustering and differential expression analysis.