Injection Valve and Sample Loop for scRNA-Seq

Sponsor: Dolomite Bio

The Injection Valve and Sample Loop for single-cell RNA sequencing workflows enables straightforward, gentle introduction of evenly distributed mRNA capture beads for individual encapsulation of cells. The Injection Valve and Sample Loop is a complete module allowing barcoded mRNA capture beads in lysis buffer to be efficiently flowed through the RNA-Seq chip without clustering or blockages. Bead damage prior to encapsulation is negligible as mechanical stirring is unnecessary, and its four-way valve, and simple, compact microfluidic connections enable seamless switching between the injection of the bead suspension and pumping of a driving fluid, with minimal dead volume and bead wastage compared to traditional syringe pump methods.